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Independent GLP-1 Testing · Elyria, Ohio

Tirzepatide, tested by an independent U.S. lab.

The long fatty acid chain on tirzepatide is the difficult part of the synthesis, and the first thing to go missing when a batch is made badly. A backbone without its side chain still behaves like a peptide on a purity trace, so the percentage can look respectable while the molecule is wrong. What it cannot do is weigh the right amount, which is why identity here is a mass measurement and not an inference from a chromatogram. Every sample is run for intact mass by LC-MS and for purity by HPLC-UV in Elyria, Ohio, and a second analyst checks the work before the certificate is signed. Identity, quantity, and purity are included in the base test. Endotoxin testing, sterility testing, and heavy metals testing are available as optional add-ons.

$195.00 per sample · signed COA
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How a sample becomes
a certificate.

Every tirzepatide sample moves through the same five steps. No shortcuts for rush orders. No unnamed hands in the middle.

Intake.

Your sample arrives. We photograph it, weigh it, log every detail, and assign a tracking number that follows it onto every page.

Prep.

A named analyst prepares the run: dissolutions, dilutions, internal standards. Their initials go in the log.

Run.

HPLC-UV for purity, LC-MS for identity, and quantitative analysis for the amount present, with system-suitability standards bracketing your sample on either side.

Review.

A second analyst reviews the chromatogram independently. Nothing ships with one set of eyes on it.

Sign.

The COA is digitally signed, hashed, and published. Anyone with the link can verify the signature against the raw data.

What we look for

What we look for in tirzepatide

The whole molecule, not just a peak.

A truncated backbone or an unattached side chain can still produce a clean-looking purity number. Intact mass is what tells you the molecule in the vial is the molecule on the label.

Whether it is tirzepatide at all.

Tirzepatide and semaglutide get confused on labels and in production. They differ by 699.87 daltons on average mass, so one run settles it.

How much is actually in the vial.

Purity and quantity answer different questions. The base test reports both HPLC-UV percent-area purity and the measured quantity in the submitted sample.

What you receive

Paper you can stake a reputation on.

01

A signed Certificate of Analysis

Identity, quantity, and purity on one document, signed by the director, not a template stamp.

02

Method-level transparency

Column, wavelength, ionization mode, and acceptance criteria are stated, not implied.

03

Public verification

Each COA carries a hash and signature anyone can check at our verify endpoint, on or off our site.

Related testing

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Frequently asked

Tirzepatide testing, answered.

Does ECA test tirzepatide samples?+
Yes. Erie Coast Analytical accepts tirzepatide research samples through the catalog and returns analytical documentation once testing is complete. Identity is confirmed by mass spectrometry, quantity is measured, and purity is assessed by HPLC-UV.
Is tirzepatide testing performed in the United States?+
Yes. All analysis is performed in-house at our laboratory in Elyria, Ohio. We are an independent U.S. lab. We don't outsource testing or ship samples overseas, and every result is produced and signed by our own analysts.
What does the tirzepatide Certificate of Analysis include?+
A completed tirzepatide COA documents identity by intact-mass LC-MS, the measured quantity, HPLC-UV purity as percent area, the method parameters used, and the analyst and director who signed off. It is digitally signed and independently verifiable.
How long does tirzepatide testing take?+
Typical turnaround is 24-48 hours from the day your sample is received at the lab. You'll get shipping instructions, a packing slip, and labels after checkout.
What methods are used for tirzepatide identity, quantity, and purity?+
Identity is assessed by LC-MS intact-mass analysis, quantity is measured separately, and purity is reported by reversed-phase HPLC-UV as percent area, with system-suitability standards run on either side of the sample.
How do I know a tirzepatide sample is not semaglutide?+
Because they weigh different amounts, and not by a little. Tirzepatide is about 700 daltons heavier than semaglutide, a gap no instrument could mistake. LC-MS measures the intact mass of what is in your vial and compares it against the expected value. A sample labelled tirzepatide that comes back at the semaglutide mass is not a purity problem, it is a different drug, and a purity-only certificate would never catch it.
Is this medical or dosing advice?+
No. ECA provides analytical testing for research compounds only. We do not provide medical advice, dosing guidance, treatment claims, or clinical evaluation of any kind.
Erie Coast Analytical provides analytical testing services for research compounds and does not provide medical advice, dosing guidance, treatment claims, or clinical evaluation. Endotoxin testing, sterility testing, and heavy metals testing are available as optional add-ons. The interactive catalog remains the source of truth for current availability, checkout pricing, and add-on testing panels.