🏭 Catalog 🏛 About 🔒 Verify 🔎 Public Tests 📦 My Orders
🛒 Cart
Independent Peptide Testing · Elyria, Ohio

TB-500, tested by an independent U.S. lab.

The name TB-500 gets applied to two different molecules: a seven residue fragment of thymosin beta-4, and the full-length 43 residue protein, which weighs more than five times as much. Anti-doping authorities use the name for the fragment. Plenty of suppliers use it for the full-length peptide. Both turn up at similar prices, sometimes from vendors who could not tell you which one they shipped. Purity testing cannot separate them, because either one can be perfectly pure. Weighing them separates them in a single run. Identity, quantity, and purity are included in the base test. Endotoxin testing, sterility testing, and heavy metals testing are available as optional add-ons.

$195.00 per sample · signed COA
Start TB-500 testing → View full catalog

How a sample becomes
a certificate.

Every TB-500 sample moves through the same five steps. No shortcuts for rush orders. No unnamed hands in the middle.

Intake.

Your sample arrives. We photograph it, weigh it, log every detail, and assign a tracking number that follows it onto every page.

Prep.

A named analyst prepares the run: dissolutions, dilutions, internal standards. Their initials go in the log.

Run.

HPLC-UV for purity, LC-MS for identity, and quantitative analysis for the amount present, with system-suitability standards bracketing your sample on either side.

Review.

A second analyst reviews the chromatogram independently. Nothing ships with one set of eyes on it.

Sign.

The COA is digitally signed, hashed, and published. Anyone with the link can verify the signature against the raw data.

What we look for

What we look for in TB-500

Fragment or full length.

The acetylated fragment weighs about 889 daltons. Full-length thymosin beta-4, all 43 residues of it, weighs about 4,963. Once it is measured there is nothing to argue about, which is why it is the first thing on our certificate.

Whether the sequence matches either one.

A mass that lands between the two answers is its own kind of answer, and we would rather tell you that than round to the nearest expectation.

Purity of whichever molecule you have.

Reported as percent area by HPLC-UV, against the compound we actually identified, not the one printed on the vial.

What you receive

Paper you can stake a reputation on.

01

A signed Certificate of Analysis

Identity, quantity, and purity on one document, signed by the director, not a template stamp.

02

Method-level transparency

Column, wavelength, ionization mode, and acceptance criteria are stated, not implied.

03

Public verification

Each COA carries a hash and signature anyone can check at our verify endpoint, on or off our site.

Related testing

CJC-1295 testingWith DAC or without, often mislabelled BPC-157 testingTwo salt forms hiding behind one label GHK-Cu testingHalf peptide, half copper Ipamorelin testingIdentity, quantity, and purity Tirzepatide testingDual agonist, and the side chain is the hard part Semaglutide testingThe most copied peptide on the market Retatrutide testingNewest of the three, and the most variable
Frequently asked

TB-500 testing, answered.

Does ECA test TB-500 samples?+
Yes. Erie Coast Analytical accepts TB-500 research samples through the catalog and returns analytical documentation once testing is complete. Identity is confirmed by mass spectrometry, quantity is measured, and purity is assessed by HPLC-UV.
Is TB-500 testing performed in the United States?+
Yes. All analysis is performed in-house at our laboratory in Elyria, Ohio. We are an independent U.S. lab. We don't outsource testing or ship samples overseas, and every result is produced and signed by our own analysts.
What does the TB-500 Certificate of Analysis include?+
The TB-500 documentation reports the measured intact mass and which molecule it matches, the measured quantity, HPLC-UV purity as percent area, method parameters, and analyst and director review. If the sample does not match the label, the documentation says so.
How long does TB-500 testing take?+
Typical turnaround is 24-48 hours from the day your sample is received at the lab. You'll get shipping instructions, a packing slip, and labels after checkout.
What methods are used for TB-500 identity, quantity, and purity?+
Identity is confirmed by LC-MS intact-mass analysis. Quantity is measured and reported separately from percent-area purity. Purity is measured by reversed-phase HPLC-UV and reported as percent area.
My vendor says TB-500 and thymosin beta-4 are the same thing. Are they?+
No. One is a seven residue piece of the other. The full-length protein weighs more than five times what the fragment weighs, so a milligram of one holds a very different number of molecules than a milligram of the other. Vendors use the names interchangeably. The instrument does not.
Is this medical or dosing advice?+
No. ECA provides analytical testing for research compounds only. We do not provide medical advice, dosing guidance, treatment claims, or clinical evaluation of any kind.
Erie Coast Analytical provides analytical testing services for research compounds and does not provide medical advice, dosing guidance, treatment claims, or clinical evaluation. Endotoxin testing, sterility testing, and heavy metals testing are available as optional add-ons. The interactive catalog remains the source of truth for current availability, checkout pricing, and add-on testing panels.